| Gene | Smc3 |
| Chromosome | 19 |
| Deletion Start | 53,631,633 |
| Deletion End | 53,600,543 |
| Deletion Size | 31,091 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,601,251 | 53,601,270 | AAGCCGCTGGGTTTGGAGAG |
| TUP | 53,601,272 | 53,601,292 | TTCTCCCAGAGCAGCCCTGCA |
| TUR | 53,601,331 | 53,601,354 | CGGGAGCAAGCAAGTTAAAGATTC |
| TDF | 53,631,478 | 53,631,501 | TTGTTGCAGGATGCTATTCCTATG |
| TDP | 53,631,504 | 53,631,525 | CAGTAAGCTGAGGTACAATCCC |
| TDR | 53,631,565 | 53,631,584 | TCCATGCTCCGACAGATGAG |
PCR Primers
| Primer | Sequence |
| SU | TCCGCATCGCTTCCATCG |
| SD | GAAATTTTGGTAGTTAGCTGGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17956C-A9 | VGB6 | C57BL/6NTac | |
| 17956C-C11 | VGB6 | C57BL/6NTac | |
| 17956C-E10 | VGB6 | C57BL/6NTac | |
