| Gene | Taf2 |
| Chromosome | 15 |
| Deletion Start | 55,071,926 |
| Deletion End | 55,040,365 |
| Deletion Size | 31,562 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,071,897 | 55,071,917 | GGCTTAGAGTCCTCCAGGATG |
| TUP | 55,071,867 | 55,071,894 | CAGGAAGAAAGGAGACAAGGGCTTCGAA |
| TUR | 55,071,806 | 55,071,824 | TCAGAGCAGGGCGAGAAAC |
| TDF | 55,040,845 | 55,040,864 | GGCAAGGCAGTAAGCAACAC |
| TDP | 55,040,809 | 55,040,838 | ATCCAGAGTAGACAGTGAAGGTCTGGGTTA |
| TDR | 55,040,783 | 55,040,803 | ACACAGTTTCAGCACTGGTTC |
PCR Primers
| Primer | Sequence |
| SU | CCAGACGAGAAATCCCAAAC |
| SD | GATGTATCAGTCTCTCTATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17957A-E12 | VGB6 | C57BL/6NTac | |
| 17957A-E4 | VGB6 | C57BL/6NTac | |
| 17957A-H1 | VGB6 | C57BL/6NTac | |
