| Gene | Tbl1x |
| Chromosome | X |
| Deletion Start | 77,659,779 |
| Deletion End | 77,641,453 |
| Deletion Size | 18,327 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,642,304 | 77,642,324 | CCATGCAACAAGCCACTTCAG |
| TUP | 77,642,331 | 77,642,358 | AGGGAGTGCCCTATGTTCACTTCTCTGG |
| TUR | 77,642,373 | 77,642,393 | TTGCCAACAGCTTTACCAGAG |
| TDF | 77,659,413 | 77,659,430 | TGGTTGGTGTGGCCTGAG |
| TDP | 77,659,441 | 77,659,470 | ATCTGCTCCACCATATAGGATCCAGTCTGT |
| TDR | 77,659,479 | 77,659,501 | CCAAGGACCATTCCCAGAAACTC |
PCR Primers
| Primer | Sequence |
| SU | GCCACTCTCGATTGTGTCAAC |
| SD | AGCCTGTTCTGGATGGAAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17958A-A7 | VGB6 | C57BL/6NTac | |
| 17958A-G7 | VGB6 | C57BL/6NTac | |
| 17958A-H8 | VGB6 | C57BL/6NTac | |
