| Gene | Zfp146 |
| Chromosome | 7 |
| Deletion Start | 30,162,612 |
| Deletion End | 30,161,671 |
| Deletion Size | 942 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,162,544 | 30,162,566 | CCTGTAAGGTATGTGGGAAACTC |
| TUP | 30,162,513 | 30,162,542 | TCAGCCACAAATCGAATCTTACAGAGCACG |
| TUR | 30,162,467 | 30,162,487 | CCGCACTCGTTACACTCAAAC |
| TDF | 30,105,819 | 30,105,839 | GCCTTATCAGTGCAGTGAGTG |
| TDP | 30,105,840 | 30,105,864 | TGGGAAGGCTTTCAGCCAGAAGTCC |
| TDR | 30,161,731 | 30,161,755 | GGGCGTTTAATGAGTATGGATTTTC |
PCR Primers
| Primer | Sequence |
| SU | TGCCTGCAGTTTGTTAGTGG |
| SD | CAAACGTCGTCTTGGTGTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 55937 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17959A-F10 | VGB6 | C57BL/6NTac | |
| 17959A-D3 | VGB6 | C57BL/6NTac | |
| 17959A-D6 | VGB6 | C57BL/6NTac | |
| 17959A-E6 | VGB6 | C57BL/6NTac | |
| 17959A-H10 | VGB6 | C57BL/6NTac | |
| 17959A-H3 | VGB6 | C57BL/6NTac | |
