| Gene | Cdk5rap3 |
| Chromosome | 11 |
| Deletion Start | 96,916,386 |
| Deletion End | 96,908,022 |
| Deletion Size | 8,365 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,916,359 | 96,916,376 | GACCAGGGCTGGAGAAAC |
| TUP | 96,916,334 | 96,916,358 | TGGGTCTTCTCTGGGATTCCTGTCC |
| TUR | 96,916,306 | 96,916,325 | ACAGAGGACGGGAGGACTTG |
| TDF | 96,908,636 | 96,908,660 | TGTCTTCTTCCTCTTCCATGATTCC |
| TDP | 96,908,607 | 96,908,634 | CCTTCCTGTGTACATGGCTTTCTCCTGG |
| TDR | 96,908,584 | 96,908,606 | ATCAACCCTACCCTGCGCTACTC |
PCR Primers
| Primer | Sequence |
| SU | CTACTTTCGAAACGCCATCG |
| SD | TACAATTGCTCAAGGCCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17961A-A3 | VGB6 | C57BL/6NTac | |
| 17961A-A11 | VGB6 | C57BL/6NTac | |
| 17961A-D6 | VGB6 | C57BL/6NTac | |
| 17961A-C11 | VGB6 | C57BL/6NTac | |
| 17961A-C7 | VGB6 | C57BL/6NTac | |
