| Gene | Kdm4b |
| Chromosome | 17 |
| Deletion Start | 56,386,276 |
| Deletion End | 56,351,660 |
| Deletion Size | 34,617 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,352,429 | 56,352,447 | TGAGGGCAGATGGACAGAC |
| TUP | 56,352,450 | 56,352,472 | CACAAGCATCTGTGGCCCGTGCT |
| TUR | 56,352,478 | 56,352,496 | TACACGGCTCGGCTCATAC |
| TDF | 56,385,742 | 56,385,761 | GCTGCTCCCTAGCTTGTCTG |
| TDP | 56,385,764 | 56,385,786 | TTTGCCAGACCCAGGCTTGCATC |
| TDR | 56,385,816 | 56,385,837 | AATCACCCAGGACCAGGTAGTC |
PCR Primers
| Primer | Sequence |
| SU | AGGCTACAAGGTCTGCATGG |
| SD | GGGCAGTACGGTCCACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17965A-D7 | VGB6 | C57BL/6NTac | |
| 17965A-D9 | VGB6 | C57BL/6NTac | |
| 17965A-B10 | VGB6 | C57BL/6NTac | |
| 17965A-F6 | VGB6 | C57BL/6NTac | |
| 17965A-H1 | VGB6 | C57BL/6NTac | |
| 17965A-A7 | VGB6 | C57BL/6NTac | |
