| Gene | Keap1 |
| Chromosome | 9 |
| Deletion Start | 21,237,706 |
| Deletion End | 21,230,687 |
| Deletion Size | 7,020 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,237,492 | 21,237,512 | CATGAACGAGCTTCGCCTGAG |
| TUP | 21,237,466 | 21,237,490 | CAGCAACTCTGTGACGTGACCCTGC |
| TUR | 21,237,405 | 21,237,422 | GAGGCCAGCACCACTTTG |
| TDF | 21,231,568 | 21,231,588 | ATGTCGCTGAGGTTGACTTTG |
| TDP | 21,231,538 | 21,231,564 | TCCTGATCCTCCTACTCCAGCTTCTCC |
| TDR | 21,231,510 | 21,231,527 | AGTAGCCACTGCCCTTCC |
PCR Primers
| Primer | Sequence |
| SU | AGTGAAGAGGTTGGAAAGCG |
| SD | GGAATTGATCACCGAGTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17966A-H2 | VGB6 | C57BL/6NTac | |
| 17966A-G8 | VGB6 | C57BL/6NTac | |
| 17966A-C12 | VGB6 | C57BL/6NTac | |
| 17966A-D4 | VGB6 | C57BL/6NTac | |
| 17966A-C10 | VGB6 | C57BL/6NTac | |
