| Gene | Unc50 |
| Chromosome | 1 |
| Deletion Start | 37,438,831 |
| Deletion End | 37,431,300 |
| Deletion Size | 7,532 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,431,418 | 37,431,437 | TTCGGCAGATGGACTTTGAG |
| TUP | 37,431,440 | 37,431,462 | TGCTGCATGGCAGATGCTCTACC |
| TUR | 37,431,498 | 37,431,519 | CCTTTGTTTGCTTCCGGTAGTG |
| TDF | 37,438,487 | 37,438,509 | GAAGACGAGTCCTGGAACCTTTG |
| TDP | 37,438,510 | 37,438,536 | TCTAGATAGGAGTGTTCACCCCTCAGC |
| TDR | 37,438,538 | 37,438,560 | GCTACATCTCACTAGGCAACCTG |
PCR Primers
| Primer | Sequence |
| SU | ATGAGGACGTCATTGCACAC |
| SD | ATGCCACCCAGGTAAGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17978A-C5 | VGB6 | C57BL/6NTac | |
| 17978A-E3 | VGB6 | C57BL/6NTac | |
| 17978A-G8 | VGB6 | C57BL/6NTac | |
| 17978A-G3 | VGB6 | C57BL/6NTac | |
| 17978A-G10 | VGB6 | C57BL/6NTac | |
| 17978A-G5 | VGB6 | C57BL/6NTac | |
