| Gene | Arpc2 |
| Chromosome | 1 |
| Deletion Start | 74,267,855 |
| Deletion End | 74,236,829 |
| Deletion Size | 31,027 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 74,237,096 | 74,237,114 | GGAAGCAGCGAGAGGACAG |
| TUP | 74,237,118 | 74,237,142 | CAAGGCGTGCCAGAGAACAGGAGAC |
| TUR | 74,237,169 | 74,237,186 | TCTCGGGCGTGACTTCTG |
| TDF | 74,267,560 | 74,267,579 | TCTGCTGGGAACATCCAGTG |
| TDP | 74,267,580 | 74,267,605 | TGCTTATGGGCTTGCCATGGTGTTAC |
| TDR | 74,267,670 | 74,267,691 | GCCCAAAGGAGACCAAACAAAG |
PCR Primers
| Primer | Sequence |
| SU | TGGTGGCTGCCTGCTACAG |
| SD | AGCCACCCTTTCCTCTTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 132 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17979A-B3 | VGB6 | C57BL/6NTac | |
| 17979B-A8 | VGB6 | C57BL/6NTac | |
