| Gene | Wdr67 |
| Chromosome | 15 |
| Deletion Start | 57,940,794 |
| Deletion End | 57,912,318 |
| Deletion Size | 28,477 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,912,541 | 57,912,562 | CGGAAACCGTAGATTGCAGTTG |
| TUP | 57,912,565 | 57,912,588 | CCAGAATCTGGTCCCTGGTCACGG |
| TUR | 57,912,603 | 57,912,624 | GCACAAGTCGTAGAGCCTTCTC |
| TDF | 57,939,855 | 57,939,874 | GTGTGGTGTTGCCTGAGTTG |
| TDP | 57,939,876 | 57,939,901 | TGCTGAAAGGACTGTCAGCCCTTGCT |
| TDR | 57,939,917 | 57,939,937 | CCTGCACTGTGTGAGGTCAAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTTGGGTCGCAGAATTAG |
| SD | CGCCACGACAGCTTGATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17982A-E9 | VGB6 | C57BL/6NTac | |
| 17982A-F1 | VGB6 | C57BL/6NTac | |
| 17982A-B3 | VGB6 | C57BL/6NTac | |
