| Gene | Dhx29 |
| Chromosome | 13 |
| Deletion Start | 112,969,193 |
| Deletion End | 112,927,796 |
| Deletion Size | 41,398 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,928,042 | 112,928,063 | CGTGAGCTGATGAGATCCAAAC |
| TUP | 112,928,068 | 112,928,093 | ACATCTTCCACACCTGAGCAGTGGGT |
| TUR | 112,928,154 | 112,928,173 | AGGCGAAGCCACGCTGTAAC |
| TDF | 112,968,264 | 112,968,285 | TGTAGAGTGTCCTGCGGTCTTG |
| TDP | 112,968,287 | 112,968,308 | AGCCCTGCCCTGGTGGCTTTCC |
| TDR | 112,968,342 | 112,968,364 | CCTGCCTAAACAACTGTGGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CGGAACTCTGAGACCGAATC |
| SD | CAGTGAGTTTCAGACGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17987A-C7 | VGB6 | C57BL/6NTac | |
| 17987A-D2 | VGB6 | C57BL/6NTac | |
| 17987A-H2 | VGB6 | C57BL/6NTac | |
| 17987A-F5 | VGB6 | C57BL/6NTac | |
| 17987A-F9 | VGB6 | C57BL/6NTac | |
| 17987A-C6 | VGB6 | C57BL/6NTac | |
