| Gene | Ect2 |
| Chromosome | 3 |
| Deletion Start | 27,146,003 |
| Deletion End | 27,117,783 |
| Deletion Size | 28,221 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 27,145,951 | 27,145,974 | CAGGAAGAAGGAGGAGCTTGTAAG |
| TUP | 27,145,923 | 27,145,948 | TGAGACCCTGACCTAGTAACGCAGGG |
| TUR | 27,145,880 | 27,145,903 | ACGTCACAACACCAAACAGAAATG |
| TDF | 27,118,397 | 27,118,417 | TCGGGTGGATGCTTATCTTTC |
| TDP | 27,118,374 | 27,118,396 | TGCAGCTTCCCTTTGGCAGGCTT |
| TDR | 27,118,351 | 27,118,371 | CCTGTCCTCAGGCTTGCTTAC |
PCR Primers
| Primer | Sequence |
| SU | GTGAGTGTGTGAACCTTGGG |
| SD | GAATAAGACAAGCTAAGGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17988A-E5 | VGB6 | C57BL/6NTac | |
