| Gene | Gm608 |
| Chromosome | 16 |
| Deletion Start | 44,221,842 |
| Deletion End | 44,200,635 |
| Deletion Size | 21,208 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,201,413 | 44,201,433 | GAGTCCACAGGTGGGAAGATG |
| TUP | 44,201,435 | 44,201,457 | CTCGGATGCAGTCTGCACCCTGC |
| TUR | 44,201,485 | 44,201,505 | GCTCTGCGATTCCTGATTCTG |
| TDF | 44,221,559 | 44,221,579 | CTCCCAGACCTGTTGGCTTTG |
| TDP | 44,221,581 | 44,221,605 | TCAGCCAAGTTTCCCTCTTCTCCCT |
| TDR | 44,221,606 | 44,221,624 | CGTGCATTGGTGGCATCTC |
PCR Primers
| Primer | Sequence |
| SU | TTCTCTTCCTTCATGCTGGC |
| SD | TCTCAGCTCCCACCTACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17990B-A10 | VGB6 | C57BL/6NTac | |
| 17990B-B3 | VGB6 | C57BL/6NTac | |
| 17990B-E11 | VGB6 | C57BL/6NTac | |
| 17990B-H2 | VGB6 | C57BL/6NTac | |
| 17990B-H8 | VGB6 | C57BL/6NTac | |
