| Gene | Gnb5 |
| Chromosome | 9 |
| Deletion Start | 75,340,231 |
| Deletion End | 75,311,398 |
| Deletion Size | 28,834 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,311,592 | 75,311,613 | GTGGCCGACAAGTTACATAGAG |
| TUP | 75,311,620 | 75,311,647 | TGTAGGATGAGTTCCCAAGCTCTAGGAA |
| TUR | 75,311,650 | 75,311,668 | TGCCAGGCAGACATCAGAG |
| TDF | 75,339,697 | 75,339,719 | GGTTCTTGGTACTCACTGCTTTG |
| TDP | 75,339,721 | 75,339,744 | TCATCACCCTGTAGACGGGCTTCA |
| TDR | 75,339,761 | 75,339,779 | CCAGCCTGGTTTCCTCATC |
PCR Primers
| Primer | Sequence |
| SU | CCAAGGATCCGTTGAAAGC |
| SD | TTCCTGTAGTATTTTCTGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17991A-F7 | VGB6 | C57BL/6NTac | |
| 17991A-H8 | VGB6 | C57BL/6NTac | |
