| Gene | Plekhg2 |
| Chromosome | 7 |
| Deletion Start | 28,371,220 |
| Deletion End | 28,359,756 |
| Deletion Size | 11,465 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,370,334 | 28,370,354 | CACCTGTTGCCTGTGGGAATC |
| TUP | 28,370,306 | 28,370,328 | TTCGGTGAAACCCTCCAGGCTGG |
| TUR | 28,370,264 | 28,370,282 | CATACGCCCGCTCTGTCTC |
| TDF | 28,360,682 | 28,360,700 | TCAGCCCTTTGCCAGTGTG |
| TDP | 28,360,653 | 28,360,679 | CAAGTAGCCCTGACCAGCAGATCCCAG |
| TDR | 28,360,609 | 28,360,631 | CTCCATGTCTGGGAAGTCAGTTG |
PCR Primers
| Primer | Sequence |
| SU | GTCTGTAGGACCCTGGCATC |
| SD | TCTTAAGAGCTTTGACATTTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17993A-B11 | VGB6 | C57BL/6NTac | |
| 17993B-F7 | VGB6 | C57BL/6NTac | |
| 17993B-G3 | VGB6 | C57BL/6NTac | |
