| Gene | Mis18bp1 |
| Chromosome | 12 |
| Deletion Start | 65,161,934 |
| Deletion End | 65,133,450 |
| Deletion Size | 28,485 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,161,252 | 65,161,271 | TGGGACCGAAGAGAGAGAAC |
| TUP | 65,161,225 | 65,161,251 | TGCAGACATGGTATCAAATCCCAGCAC |
| TUR | 65,161,189 | 65,161,210 | CAAACTTGAGGCCAACCTAGAC |
| TDF | 65,134,284 | 65,134,306 | AAGCAGTACCAGCCTTATTACAC |
| TDP | 65,134,259 | 65,134,282 | AAGCAAGTGACTAGACTTTTCTAA |
| TDR | 65,134,230 | 65,134,249 | GCCGTTGCCATTATCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGAATGTCAAACTGCTCGTC |
| SD | GGCTTGTAATTTCAGGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18000A-C9 | VGB6 | C57BL/6NTac | |
| 18000A-D8 | VGB6 | C57BL/6NTac | |
| 18000A-E3 | VGB6 | C57BL/6NTac | |
| 18000A-C6 | VGB6 | C57BL/6NTac | |
