| Gene | Rab7l1 |
| Chromosome | 1 |
| Deletion Start | 131,872,622 |
| Deletion End | 131,867,655 |
| Deletion Size | 4,968 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,868,057 | 131,868,076 | CCGACGGGTGTTGAGGAAAG |
| TUP | 131,868,080 | 131,868,104 | CCCGTTACCTTAGGAGGAAAGCCCA |
| TUR | 131,868,145 | 131,868,165 | TCATCAGACCTGACCGGAATC |
| TDF | 131,872,483 | 131,872,506 | TGATGAACAATTCCAGGGAAGATG |
| TDP | 131,872,507 | 131,872,532 | TGATGTCTTTGTCCACCCAAGGGAAC |
| TDR | 131,872,535 | 131,872,555 | GGCTTGGCTTGGAGATTGATG |
PCR Primers
| Primer | Sequence |
| SU | CTGAAAGGAAGGTGCCAATC |
| SD | TACATCAGGAGCAATGTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18002A-A4 | VGB6 | C57BL/6NTac | |
| 18002A-A7 | VGB6 | C57BL/6NTac | |
| 18002A-C2 | VGB6 | C57BL/6NTac | |
| 18002A-E9 | VGB6 | C57BL/6NTac | |
| 18002A-H2 | VGB6 | C57BL/6NTac | |
| 18002A-E4 | VGB6 | C57BL/6NTac | |
