| Gene | Dhfr |
| Chromosome | 13 |
| Deletion Start | 92,384,809 |
| Deletion End | 92,355,262 |
| Deletion Size | 29,548 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,355,309 | 92,355,328 | TGGCAAGAACGGAGACCTAC |
| TUP | 92,355,330 | 92,355,352 | CTGGCCTCCGCTCAGGTATTGGC |
| TUR | 92,355,379 | 92,355,400 | TGCATGACCCTACTCAGTGAAC |
| TDF | 92,384,512 | 92,384,532 | GGTTGCACTTTCTGGCTTCAC |
| TDP | 92,384,535 | 92,384,563 | AGTTTACTGTTTCCTTCTGCCACCTGTGT |
| TDR | 92,384,583 | 92,384,605 | CTTTCTTCAACATGGCAAGTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTACCTTCTCTGCTGGCTCC |
| SD | CCACTTGAGGTCTCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18004A-B2 | VGB6 | C57BL/6NTac | |
| 18004A-B10 | VGB6 | C57BL/6NTac | |
| 18004A-C12 | VGB6 | C57BL/6NTac | |
| 18004A-D8 | VGB6 | C57BL/6NTac | |
