| Gene | Gen1 |
| Chromosome | 12 |
| Deletion Start | 11,262,564 |
| Deletion End | 11,241,223 |
| Deletion Size | 21,342 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,262,050 | 11,262,068 | TGTCCCGATGTCTGCCAAG |
| TUP | 11,262,023 | 11,262,048 | TCATGTCCTGGCAGTATCTCTGCTGG |
| TUR | 11,261,979 | 11,262,002 | GAAGAGGTTAAGAACAGGCAAAGG |
| TDF | 11,242,067 | 11,242,088 | GAACAAGGGACCTCAGACAGTG |
| TDP | 11,242,042 | 11,242,066 | AAGGGTCCGGTAGAGACCTTCAGCA |
| TDR | 11,242,019 | 11,242,038 | GGAGCTGCTCTTCAGGGTTG |
PCR Primers
| Primer | Sequence |
| SU | TTTCCACTGTGAACTGTATAGCC |
| SD | TTGAAGCTGTCACCAGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18005A-B4 | VGB6 | C57BL/6NTac | |
| 18005A-G3 | VGB6 | C57BL/6NTac | |
