18007   Otop3     Status: ES cell colonies screened / QC positives

GeneOtop3
Chromosome11
Deletion Start115,346,499
Deletion End115,334,887
Deletion Size11,613
Genome Build38
MGI IDMGI:1916852
NCBI Gene ID69602
BAC ID136C18
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF115,335,046115,335,066CAAGGATTGGACAGGGCAGAG
TUP115,335,067115,335,092TGAGTGGGACGACCCTAAGCTCTTCC
TUR115,335,115115,335,136GGAGAAGGATGTAGGCGCAGAG
TDF115,346,081115,346,103GAGAAAGACTCTTGGCTGTGAAC
TDP115,346,105115,346,125CGGCTCTGCAAGGCCCAGGAT
TDR115,346,158115,346,177GCGAGGCTGACCTAGAACTG

PCR Primers

PrimerSequence
SUCCGCCTCTCTGCCTCGTC
SDTCCCACAGAAGGACTCAACC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR91 bp++-
TDFTDR97 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
18007A-A1VGB6C57BL/6NTac
18007A-D6VGB6C57BL/6NTac
18007A-H3VGB6C57BL/6NTac
18007A-H12VGB6C57BL/6NTac
18007A-D2VGB6C57BL/6NTac
18007A-D7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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