| Gene | Got2 |
| Chromosome | 8 |
| Deletion Start | 95,888,302 |
| Deletion End | 95,865,123 |
| Deletion Size | 23,180 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,887,771 | 95,887,789 | CGCGCATGTCCTTGACCAG |
| TUP | 95,887,749 | 95,887,770 | CTGCCCTGCGGGACAACAGGTC |
| TUR | 95,887,689 | 95,887,708 | GTCACGCACGCTGCTTCTAC |
| TDF | 95,865,249 | 95,865,271 | TAGCTCTTTTCTTTCTGCAGGTA |
| TDP | 95,865,224 | 95,865,247 | AGCGGCTGACCAAGGAGTTCTCGG |
| TDR | 95,865,201 | 95,865,222 | TCGGCCATCCTTTGTCATGTAG |
PCR Primers
| Primer | Sequence |
| SU | CCTTCTTTCAAACTGGCACG |
| SD | GACAGGTGTTTGGTGCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18009A-A11 | VGB6 | C57BL/6NTac | |
| 18009A-C3 | VGB6 | C57BL/6NTac | |
| 18009A-E11 | VGB6 | C57BL/6NTac | |
