18010   Meaf6     Status: ES cell colonies screened / QC positives

GeneMeaf6
Chromosome4
Deletion Start125,109,678
Deletion End125,085,180
Deletion Size24,499
Genome Build38
MGI IDMGI:1917338
NCBI Gene ID70088
BAC ID194G10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,085,761125,085,778AGCAACCGGCATCCACTG
TUP125,085,783125,085,804CGGCCCTTCCAGAGCTAGCAGG
TUR125,085,805125,085,829TCTCTTCTACATCAACTGCTTCCTC
TDF125,109,495125,109,519TCATCATTAGTAGAACAGCGGGAAG
TDP125,109,520125,109,543ACCTGTCCTCCGTCCCAGTCCCTG
TDR125,109,555125,109,577TCTAAGAAGGGAGAAATGCACAG

PCR Primers

PrimerSequence
SUAGGAGATCTGCTTGCCTGTG
SDGAAGGAGCACATGAGAAGGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR69 bp++-
TDFTDR83 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
18010A-B6VGB6C57BL/6NTac
18010A-C3VGB6C57BL/6NTac
18010A-F3VGB6C57BL/6NTac
18010A-F5VGB6C57BL/6NTac
18010A-E7VGB6C57BL/6NTac
18010A-E2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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