| Gene | Pole4 |
| Chromosome | 6 |
| Deletion Start | 82,652,836 |
| Deletion End | 82,647,962 |
| Deletion Size | 4,875 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,652,029 | 82,652,047 | AGCTATGGGCTGGGCTTTC |
| TUP | 82,651,997 | 82,652,024 | AGCTGTTTCACAAGGCATCATTGAAGCA |
| TUR | 82,651,899 | 82,651,920 | TGGCAGCATAGAAGGGAATGAG |
| TDF | 82,648,290 | 82,648,314 | TGGTGATTCTTTAGCTCTGTTCCAG |
| TDP | 82,648,250 | 82,648,277 | TTCTGTGCATTGGCAGGGTTACTTACCA |
| TDR | 82,648,217 | 82,648,241 | CCAAGGGAGATGTCATTAGAGAAGG |
PCR Primers
| Primer | Sequence |
| SU | AAACCGCACTTCCAATTCTG |
| SD | GTCTGTGCCTGATCACATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 149 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18012A-G8 | VGB6 | C57BL/6NTac | |
| 18012A-F2 | VGB6 | C57BL/6NTac | |
| 18012A-G4 | VGB6 | C57BL/6NTac | |
| 18012A-F3 | VGB6 | C57BL/6NTac | |
| 18012A-C12 | VGB6 | C57BL/6NTac | |
