| Gene | Rundc3a |
| Chromosome | 11 |
| Deletion Start | 102,402,201 |
| Deletion End | 102,393,679 |
| Deletion Size | 8,523 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,394,321 | 102,394,343 | CCAATGAAGGTCGAGAGCACTAC |
| TUP | 102,394,344 | 102,394,365 | CCCGCTGTAGGGAGCACCCAAG |
| TUR | 102,394,393 | 102,394,412 | TCTGGGCTCCCAGTCTTTAC |
| TDF | 102,401,345 | 102,401,364 | AACGGCTTGCCTGACCAATG |
| TDP | 102,401,370 | 102,401,393 | CCCTTTCTTTGGCTGCCAGGAAGC |
| TDR | 102,401,395 | 102,401,416 | GGGAGCCACTAATTTGGTTCTG |
PCR Primers
| Primer | Sequence |
| SU | CAGTCTCCGTTGTTTCTCCG |
| SD | CAGGGTTAGACCAGAGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18013A-A5 | VGB6 | C57BL/6NTac | |
| 18013A-A9 | VGB6 | C57BL/6NTac | |
| 18013A-H10 | VGB6 | C57BL/6NTac | |
| 18013A-D3 | VGB6 | C57BL/6NTac | |
| 18013A-F5 | VGB6 | C57BL/6NTac | |
