| Gene | Ruvbl1 |
| Chromosome | 6 |
| Deletion Start | 88,497,392 |
| Deletion End | 88,465,531 |
| Deletion Size | 31,862 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,465,774 | 88,465,793 | TCACGGCACGCAAATTAGAC |
| TUP | 88,465,796 | 88,465,818 | CACCGGAAACGCAGTGGAAGGCT |
| TUR | 88,465,854 | 88,465,875 | GGCCCAAAGAACCCATATCAAC |
| TDF | 88,496,426 | 88,496,448 | CAGACGGAAGGCATCAACATCAG |
| TDP | 88,496,449 | 88,496,470 | CGAGGAGGCGCTGAACCACCTC |
| TDR | 88,496,495 | 88,496,513 | GCCACTTGGCTCACCTCAG |
PCR Primers
| Primer | Sequence |
| SU | CTCAGTCAAATGTGGCCAGG |
| SD | GGGATAGAACCAAGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18014A-B7 | VGB6 | C57BL/6NTac | |
| 18014A-B9 | VGB6 | C57BL/6NTac | |
| 18014A-C4 | VGB6 | C57BL/6NTac | |
| 18014A-C5 | VGB6 | C57BL/6NTac | |
| 18014A-F9 | VGB6 | C57BL/6NTac | |
