| Gene | Cyb561 |
| Chromosome | 11 |
| Deletion Start | 105,937,824 |
| Deletion End | 105,935,355 |
| Deletion Size | 2,470 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,937,772 | 105,937,792 | GCCGTACTATGTGGCCTTCTC |
| TUP | 105,937,749 | 105,937,771 | CCAGCTGCTGGGCCTCACTGTAG |
| TUR | 105,937,689 | 105,937,708 | GGGAGCTTTCCCAGGCAATG |
| TDF | 105,936,199 | 105,936,223 | AGCTGGTGTGGGATCTTAGTCTTTG |
| TDP | 105,936,169 | 105,936,195 | TGTACTTTGTGCAGGTGAGTCTAGTGC |
| TDR | 105,936,131 | 105,936,150 | TTGCCCTCCCTTTACCTCTC |
PCR Primers
| Primer | Sequence |
| SU | TTTCAAACTTGTGCCTTCCC |
| SD | GTCTAGCTCCAATAGGGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18018A-B2 | VGB6 | C57BL/6NTac | |
| 18018A-B6 | VGB6 | C57BL/6NTac | |
| 18018A-B10 | VGB6 | C57BL/6NTac | |
| 18018A-G9 | VGB6 | C57BL/6NTac | |
| 18018A-F9 | VGB6 | C57BL/6NTac | |
| 18018A-H7 | VGB6 | C57BL/6NTac | |
