| Gene | Gys1 |
| Chromosome | 7 |
| Deletion Start | 45,455,452 |
| Deletion End | 45,435,056 |
| Deletion Size | 20,397 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,435,698 | 45,435,716 | AAGGAGCCCAGGGCCTAAG |
| TUP | 45,435,718 | 45,435,743 | CCTGGCTTCCTATGTAGTGAGGTGCC |
| TUR | 45,435,753 | 45,435,772 | TTCCAGCTTTCCCAGTCCAC |
| TDF | 45,455,263 | 45,455,282 | GGCAGCAAGAGAAGCAACTC |
| TDP | 45,455,285 | 45,455,305 | TGGACACTGGGCCCTCCAGCT |
| TDR | 45,455,331 | 45,455,352 | ACCCAGGGAACTGGTAGGACTC |
PCR Primers
| Primer | Sequence |
| SU | CACCAATCACTTCCATGCTG |
| SD | AACATGAGGTGGTCCCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18020B-A2 | VGB6 | C57BL/6NTac | |
| 18020B-B6 | VGB6 | C57BL/6NTac | |
| 18020B-C2 | VGB6 | C57BL/6NTac | |
| 18020B-H9 | VGB6 | C57BL/6NTac | |
| 18020B-F2 | VGB6 | C57BL/6NTac | |
