| Gene | Mapk8ip2 |
| Chromosome | 15 |
| Deletion Start | 89,461,720 |
| Deletion End | 89,454,224 |
| Deletion Size | 7,497 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 89,454,291 | 89,454,308 | CATTCGGCGGGTCTCATC |
| TUP | 89,454,309 | 89,454,332 | TTCACGTAGGTCTCTTCGGCCTGC |
| TUR | 89,454,347 | 89,454,366 | TGGCCTGGGTAGTTGAAGTC |
| TDF | 89,461,401 | 89,461,418 | TTGCCTGCCACGTCTTTG |
| TDP | 89,461,421 | 89,461,446 | TCCCAGGAGTCTATGAGACCAGTGGC |
| TDR | 89,461,467 | 89,461,485 | GGGCTCCTGCTCCATCTTC |
PCR Primers
| Primer | Sequence |
| SU | ACCTTTGGTCTACCAGCGTG |
| SD | TTCATCGGTTACCTTGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18022A-A5 | VGB6 | C57BL/6NTac | |
| 18022A-G11 | VGB6 | C57BL/6NTac | |
| 18022A-G10 | VGB6 | C57BL/6NTac | |
| 18022A-C7 | VGB6 | C57BL/6NTac | |
| 18022A-C2 | VGB6 | C57BL/6NTac | |
| 18022A-E8 | VGB6 | C57BL/6NTac | |
