| Gene | Ndufa8 |
| Chromosome | 2 |
| Deletion Start | 36,049,239 |
| Deletion End | 36,036,492 |
| Deletion Size | 12,748 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,048,457 | 36,048,479 | TCTGAAACCCAGCCTGTTAAATC |
| TUP | 36,048,428 | 36,048,453 | CAGCCAAAGTGCAACAGAGTGAGGAA |
| TUR | 36,048,401 | 36,048,421 | TCCTCGACAACACGGTTCTAC |
| TDF | 36,036,952 | 36,036,971 | GGGTCCACCCTTCTCTGTTG |
| TDP | 36,036,932 | 36,036,951 | TCATTGGGCAGCAGGCAGCA |
| TDR | 36,036,870 | 36,036,890 | TGGGACTCACTCACTGGTTAG |
PCR Primers
| Primer | Sequence |
| SU | TGAAAGTGGAGACATGGACG |
| SD | GCTCAGACCTTTCGTTGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18023A-A1 | VGB6 | C57BL/6NTac | |
| 18023A-D11 | VGB6 | C57BL/6NTac | |
| 18023A-F5 | VGB6 | C57BL/6NTac | |
| 18023A-G1 | VGB6 | C57BL/6NTac | |
| 18023A-G9 | VGB6 | C57BL/6NTac | |
| 18023A-A12 | VGB6 | C57BL/6NTac | |
