| Gene | Pde6d |
| Chromosome | 1 |
| Deletion Start | 86,582,346 |
| Deletion End | 86,543,446 |
| Deletion Size | 38,901 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,581,704 | 86,581,723 | GACGGACCCCATCTTAACAC |
| TUP | 86,581,674 | 86,581,701 | TGTTGGCCTTTGGAGCATTAACATCTCA |
| TUR | 86,581,651 | 86,581,672 | CTCATCTCGCTGGAAAGAGAAC |
| TDF | 86,543,850 | 86,543,869 | CCCGAGTGCTGAGACTGAAG |
| TDP | 86,543,823 | 86,543,846 | CGCACCGCTATGCCTGGCTTTGTT |
| TDR | 86,543,754 | 86,543,775 | AAGGCCAATGCAGGAGGATCTG |
PCR Primers
| Primer | Sequence |
| SU | GCTACTCAAGATGCCAATCG |
| SD | CATCGGCATGCTAAAGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18024A-D2 | VGB6 | C57BL/6NTac | |
| 18024A-H11 | VGB6 | C57BL/6NTac | |
