| Gene | Pnrc1 |
| Chromosome | 4 |
| Deletion Start | 33,248,394 |
| Deletion End | 33,246,013 |
| Deletion Size | 2,382 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,247,563 | 33,247,582 | GCTGAGTCGGACTGCTGTTG |
| TUP | 33,247,541 | 33,247,560 | AAGTTGGCAGGAGTCTCCGT |
| TUR | 33,247,521 | 33,247,538 | ACCTTCGCGTCGGGCATC |
| TDF | 33,246,921 | 33,246,941 | TGTTAGGATTCGGGCTGTAGC |
| TDP | 33,246,899 | 33,246,920 | CCCGGCTGGCCTGGAATTTACC |
| TDR | 33,246,818 | 33,246,838 | TCCCGTCTGCCTTTCAGATTC |
PCR Primers
| Primer | Sequence |
| SU | CTCGGGTAACAACACTGGC |
| SD | CGGGAAGCTTTCTAGGAATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 124 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18025A-A1 | VGB6 | C57BL/6NTac | |
| 18025A-D6 | VGB6 | C57BL/6NTac | |
| 18025A-E9 | VGB6 | C57BL/6NTac | |
| 18025A-F6 | VGB6 | C57BL/6NTac | |
