| Gene | Prim1 |
| Chromosome | 10 |
| Deletion Start | 128,029,790 |
| Deletion End | 128,015,252 |
| Deletion Size | 14,539 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,015,271 | 128,015,290 | AGCTGCCGGAGCTACTCAAG |
| TUP | 128,015,297 | 128,015,320 | TACCGAAGGCTCTTTCCCTACGCC |
| TUR | 128,015,338 | 128,015,358 | CCATCACCTCCGCCATAGTTG |
| TDF | 128,029,500 | 128,029,521 | TTAGGTCCTGAGGCTTGAACTC |
| TDP | 128,029,525 | 128,029,548 | TCTCCAGACTTGGCAGCAAGCACC |
| TDR | 128,029,555 | 128,029,574 | GGATGGCAAGACTGCTTGTG |
PCR Primers
| Primer | Sequence |
| SU | CAATATGCTAAAGGTCTCTCC |
| SD | CTCTGCGGAGTAACGGGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18026A-D9 | VGB6 | C57BL/6NTac | |
| 18026A-F6 | VGB6 | C57BL/6NTac | |
| 18026A-G3 | VGB6 | C57BL/6NTac | |
