18028   Acp6     Status: ES cell colonies screened / QC positives

GeneAcp6
Chromosome3
Deletion Start97,176,468
Deletion End97,159,171
Deletion Size17,298
Genome Build38
MGI IDMGI:1931010
NCBI Gene ID66659
BAC ID374B3
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFACCAGAGGGAAACAACAGTAGAC
TUPCCCTCAGGCTGTGGCCTGGAGT
TURTGGGATGAGCATGGGATGATG
TDFTGGCTAACCCAGCATTATTGATG
TDPTCTAGGAGAGGAACTGTGTACTCTGCAGG
TDRTTCTGGCCTTGAGCTGAAAGAG

PCR Primers

PrimerSequence
SUCGGACGAAGTCTCAGGACTC
SDAGTTCTTAGAGTCCCAAAGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
18028A-A5VGB6C57BL/6NTac
18028A-C3VGB6C57BL/6NTac
18028A-D3VGB6C57BL/6NTac
18028A-F7VGB6C57BL/6NTac
18028A-E12VGB6C57BL/6NTac
18028A-G3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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