| Gene | Ckap2 |
| Chromosome | 8 |
| Deletion Start | 22,185,789 |
| Deletion End | 22,168,648 |
| Deletion Size | 17,142 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,185,389 | 22,185,413 | GCAGCAAGAATGTGGAAAACTTATG |
| TUP | 22,185,356 | 22,185,383 | TGGTCAGAATTTGGCGACTGGTATCAGA |
| TUR | 22,185,294 | 22,185,316 | CGAGTGAAAATTGCAGGGAACAC |
| TDF | 22,168,737 | 22,168,755 | GACCGACGTAGCGGAAGAG |
| TDP | 22,168,710 | 22,168,732 | AGGAGTCCCGAGTCCTGAGCAGT |
| TDR | 22,168,682 | 22,168,700 | GCAGGGCCACAGGAGTAAC |
PCR Primers
| Primer | Sequence |
| SU | AGCCTTATCCCTAAGGAGCG |
| SD | GCCATAGGCAGCTAACTATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 120 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18030A-D3 | VGB6 | C57BL/6NTac | |
| 18030A-E9 | VGB6 | C57BL/6NTac | |
| 18030A-F10 | VGB6 | C57BL/6NTac | |
| 18030A-H12 | VGB6 | C57BL/6NTac | |
| 18030A-D8 | VGB6 | C57BL/6NTac | |
