| Gene | Pbk |
| Chromosome | 14 |
| Deletion Start | 65,817,508 |
| Deletion End | 65,809,238 |
| Deletion Size | 8,271 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,809,431 | 65,809,451 | CCGGACTTTCTGTCTACTCAC |
| TUP | 65,809,452 | 65,809,481 | TGATGGTCGTTCTGGAATTTAGGACCTGTC |
| TUR | 65,809,484 | 65,809,502 | CAAGCCAGAGGACCACTAC |
| TDF | 65,816,641 | 65,816,662 | AAACCCAAGGAAGCGTTGGAAG |
| TDP | 65,816,667 | 65,816,695 | TGGCATCATTACTGACAAGGCAGATGTGT |
| TDR | 65,816,699 | 65,816,720 | TCCCACAGAGTAAGGCCAAAAG |
PCR Primers
| Primer | Sequence |
| SU | CTGTGATAAAATGATGTTATGGG |
| SD | GATAATGCTGAGGTGCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18033A-A2 | VGB6 | C57BL/6NTac | |
| 18033A-B1 | VGB6 | C57BL/6NTac | |
| 18033A-B4 | VGB6 | C57BL/6NTac | |
| 18033A-G8 | VGB6 | C57BL/6NTac | |
| 18033A-H6 | VGB6 | C57BL/6NTac | |
