| Gene | BC027231 |
| Chromosome | 16 |
| Deletion Start | 44,736,191 |
| Deletion End | 44,724,366 |
| Deletion Size | 11,826 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,724,598 | 44,724,620 | TGGATAGTGTGGCATGAGGATTG |
| TUP | 44,724,621 | 44,724,640 | CTGGCAAGCGCCTGGGCTTC |
| TUR | 44,724,655 | 44,724,673 | AGTCGTGCTCTCCTGTTCC |
| TDF | 44,735,747 | 44,735,767 | TCTCTCTGCAACTGCCTTCTC |
| TDP | 44,735,768 | 44,735,791 | CGAGGCTCTCGTACCAGCACTTCA |
| TDR | 44,735,835 | 44,735,856 | CTCTGCGGTTTCCTTTGTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GCATAGCTTCGAAGTTGCTC |
| SD | GCTTTGCTACTCCAGCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18035A-A7 | VGB6 | C57BL/6NTac | |
| 18035A-A10 | VGB6 | C57BL/6NTac | |
| 18035A-B1 | VGB6 | C57BL/6NTac | |
| 18035A-C1 | VGB6 | C57BL/6NTac | |
| 18035A-F11 | VGB6 | C57BL/6NTac | |
| 18035A-C9 | VGB6 | C57BL/6NTac | |
