| Gene | Fabp12 |
| Chromosome | 3 |
| Deletion Start | 10,252,306 |
| Deletion End | 10,245,997 |
| Deletion Size | 6,310 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,251,880 | 10,251,902 | GGACCTTTGCTTTCTCACCTCTG |
| TUP | 10,251,858 | 10,251,878 | TGGCTGCTGGCTTTCCTTGGA |
| TUR | 10,251,827 | 10,251,845 | CATGGCGGCAAGGGTAGTG |
| TDF | 10,246,594 | 10,246,613 | AGGCCAACATGGTTCAATGG |
| TDP | 10,246,547 | 10,246,576 | TTAGTTCACTGAGAAGAATGCCAGACACCA |
| TDR | 10,246,500 | 10,246,522 | GAGGTGAAATGACTTGCCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGGTGCTATGTATGCATACTC |
| SD | GAGGTCCGCTGTGGATAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18036A-H12 | VGB6 | C57BL/6NTac | |
| 18036A-B1 | VGB6 | C57BL/6NTac | |
| 18036A-E3 | VGB6 | C57BL/6NTac | |
| 18036A-F2 | VGB6 | C57BL/6NTac | |
| 18036A-C6 | VGB6 | C57BL/6NTac | |
