|
|
Sequence Downloads and Strategy Information
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,048,676 | 73,048,696 | TTTGCCTGTGGTTTGGTAGTC |
| TUP | 73,048,703 | 73,048,729 | CCCTTCTTAAAGCTGGTCTGAGGCCTG |
| TUR | 73,048,746 | 73,048,766 | GCAGGGAGTTCACGTTGTATG |
| TDF | 73,078,830 | 73,078,850 | CACCTCTCCCGATGTTTCCTG |
| TDP | 73,078,851 | 73,078,876 | CAGAGCAGGTGTTGGCAGTGTCCTTA |
| TDR | 73,078,885 | 73,078,906 | GATGCCGAGTACCAAGTAGTTC |
PCR Primers
| Primer | Sequence |
| SU | GCCTAGATGATAGATCGCGG |
| SD | TTCTCGGCTCTTTGGATGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the KOMP Repository.| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18038A-E10 | VGB6 | C57BL/6NTac | |
| 18038A-H2 | VGB6 | C57BL/6NTac |
