| Gene | Arid2 |
| Chromosome | 15 |
| Deletion Start | 96,378,950 |
| Deletion End | 96,356,848 |
| Deletion Size | 22,103 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,357,699 | 96,357,719 | GCGTAGCCTAGACATCACCTG |
| TUP | 96,357,721 | 96,357,750 | TGTGTTTTGATGGTGTACTCATGCCCTGTA |
| TUR | 96,357,772 | 96,357,792 | GAGGGTCACACGCACACTATG |
| TDF | 96,378,814 | 96,378,835 | AACACTCCTATGCCACCTTCAC |
| TDP | 96,378,837 | 96,378,861 | AGCTGTACAAGTGCAGGGCCAGCCT |
| TDR | 96,378,881 | 96,378,899 | TGACTGGACGCACTGACTG |
PCR Primers
| Primer | Sequence |
| SU | TTGCTATGGCTTCATTGGTG |
| SD | TACTAGCATGAAGCAATCGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18040A-A9 | VGB6 | C57BL/6NTac | |
| 18040A-G8 | VGB6 | C57BL/6NTac | |
| 18040A-C11 | VGB6 | C57BL/6NTac | |
| 18040A-E10 | VGB6 | C57BL/6NTac | |
| 18040A-D7 | VGB6 | C57BL/6NTac | |
| 18040A-B10 | VGB6 | C57BL/6NTac | |
