| Gene | Rnaseh2b |
| Chromosome | 14 |
| Deletion Start | 62,372,530 |
| Deletion End | 62,332,119 |
| Deletion Size | 40,412 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,332,502 | 62,332,523 | CAAACCAGAGGTCGCAGTAGTC |
| TUP | 62,332,525 | 62,332,546 | CATGCTGAGCCGCTCTGCTGCT |
| TUR | 62,332,589 | 62,332,610 | TCAAACCACGCTCACATAAACC |
| TDF | 62,372,228 | 62,372,246 | GATCCTGAGTGGGCAGCAG |
| TDP | 62,372,252 | 62,372,274 | TTTGAGCTCTGCCACTCACGTGC |
| TDR | 62,372,287 | 62,372,308 | GCAAATCACACAAGCCTGAATC |
PCR Primers
| Primer | Sequence |
| SU | AGCCAATTTGAAGGCTGCTC |
| SD | CAGGACAGAGCAGGGAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18045A-G2 | VGB6 | C57BL/6NTac | |
| 18045A-G5 | VGB6 | C57BL/6NTac | |
| 18045A-H9 | VGB6 | C57BL/6NTac | |
