| Gene | |
| Chromosome | 14 |
| Deletion Start | 20,693,801 |
| Deletion End | 20,675,989 |
| Deletion Size | 17,813 |
| Genome Build | 38 |
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|
Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,676,351 | 20,676,371 | CCAGATTCAGCAACAGCTCAC |
| TUP | 20,676,377 | 20,676,399 | TGGGTGTCACCCTGGACAGCATC |
| TUR | 20,676,418 | 20,676,437 | CCCTTCCATCCAGCTCTCAG |
| TDF | 20,692,812 | 20,692,832 | TGGGCTCAACCTCTTTGTCTG |
| TDP | 20,692,835 | 20,692,857 | TGGGAGCAAGCGTCCAACAAGGT |
| TDR | 20,692,887 | 20,692,907 | CCGCTGGTGATCTGACTGAAG |
PCR Primers
| Primer | Sequence |
| SU | AATGGCTGCTGCTGTTCTG |
| SD | TCCAAAGGGACACACTGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18046A-A3 | VGB6 | C57BL/6NTac | |
| 18046B-A10 | VGB6 | C57BL/6NTac | |
| 18046B-E5 | VGB6 | C57BL/6NTac | |
| 18046B-H10 | VGB6 | C57BL/6NTac | |
