| Gene | Crnkl1 |
| Chromosome | 2 |
| Deletion Start | 145,934,601 |
| Deletion End | 145,918,350 |
| Deletion Size | 16,252 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 145,933,662 | 145,933,681 | GGGCTCTGGTGTAGTCTGTG |
| TUP | 145,933,635 | 145,933,661 | AGAGCAGACTGAGCACAGCTGGTATGC |
| TUR | 145,933,607 | 145,933,628 | GAATGGAAGACACGGCAGGAAG |
| TDF | 145,918,867 | 145,918,885 | GTGGAGCCGCTGTGCTTAG |
| TDP | 145,918,837 | 145,918,863 | TTTAGCTTCCTCCCACTTGGTCCTTCA |
| TDR | 145,918,790 | 145,918,809 | CACCGGGCAGATGAGGTTAC |
PCR Primers
| Primer | Sequence |
| SU | TGAAATGGATTTCTACGCCC |
| SD | AATGGAAACGTACAGCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18049A-F7 | VGB6 | C57BL/6NTac | |
| 18049A-B1 | VGB6 | C57BL/6NTac | |
| 18049A-H3 | VGB6 | C57BL/6NTac | |
| 18049A-E10 | VGB6 | C57BL/6NTac | |
| 18049A-C10 | VGB6 | C57BL/6NTac | |
