Gene | Crnkl1 |
Chromosome | 2 |
Deletion Start | 145,934,601 |
Deletion End | 145,918,350 |
Deletion Size | 16,252 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 145,933,662 | 145,933,681 | GGGCTCTGGTGTAGTCTGTG |
TUP | 145,933,635 | 145,933,661 | AGAGCAGACTGAGCACAGCTGGTATGC |
TUR | 145,933,607 | 145,933,628 | GAATGGAAGACACGGCAGGAAG |
TDF | 145,918,867 | 145,918,885 | GTGGAGCCGCTGTGCTTAG |
TDP | 145,918,837 | 145,918,863 | TTTAGCTTCCTCCCACTTGGTCCTTCA |
TDR | 145,918,790 | 145,918,809 | CACCGGGCAGATGAGGTTAC |
PCR Primers
Primer | Sequence |
SU | TGAAATGGATTTCTACGCCC |
SD | AATGGAAACGTACAGCCACC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 75 bp | + | + | - |
TDF | TDR | 96 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
18049A-F7 | VGB6 | C57BL/6NTac | |
18049A-B1 | VGB6 | C57BL/6NTac | |
18049A-H3 | VGB6 | C57BL/6NTac | |
18049A-E10 | VGB6 | C57BL/6NTac | |
18049A-C10 | VGB6 | C57BL/6NTac | |