| Gene | Dusp9 |
| Chromosome | X |
| Deletion Start | 73,642,453 |
| Deletion End | 73,640,231 |
| Deletion Size | 2,223 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,640,731 | 73,640,749 | GGATGCTGACTCGGTGCTG |
| TUP | 73,640,752 | 73,640,773 | CGTCCTGCTCCAGAAGCTGCGG |
| TUR | 73,640,805 | 73,640,824 | GGCCACTGACCCTCACTTAC |
| TDF | 73,641,499 | 73,641,516 | TCTGAGGCTGACCGTGAC |
| TDP | 73,641,518 | 73,641,544 | CCTTGAGCTGTGGCCTAGATTCTGAGA |
| TDR | 73,641,579 | 73,641,598 | GCAGGATCTGGACTGGGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTTCAGAGACCGGTAGACGC |
| SD | GGTTCCTGTGGCAAAGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18050A-D11 | VGB6 | C57BL/6NTac | |
| 18050A-E2 | VGB6 | C57BL/6NTac | |
