| Gene | Elf4 |
| Chromosome | X |
| Deletion Start | 48,424,146 |
| Deletion End | 48,414,593 |
| Deletion Size | 9,554 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,423,601 | 48,423,622 | GGGCTCGCTCATAAATGTCTTC |
| TUP | 48,423,576 | 48,423,600 | TCGGCCTAAGGATTCAGGACATGCC |
| TUR | 48,423,531 | 48,423,554 | CCTACCGTAGTTTCCAACATCCTC |
| TDF | 48,415,343 | 48,415,362 | GACTACGGTGCTGACCAATG |
| TDP | 48,415,315 | 48,415,340 | CCTCCTGCCAGTACTACTGCTCCAAC |
| TDR | 48,415,285 | 48,415,304 | ACCTGCGGGACACTCTGAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCCTCTTGGCTTCACTAGC |
| SD | GGACAGACGGACACACACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18051A-D7 | VGB6 | C57BL/6NTac | |
| 18051A-D12 | VGB6 | C57BL/6NTac | |
