| Gene | Morn5 |
| Chromosome | 2 |
| Deletion Start | 36,079,646 |
| Deletion End | 36,049,532 |
| Deletion Size | 30,115 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,050,215 | 36,050,236 | GTCAGAATCCCTGCAAACACAG |
| TUP | 36,050,237 | 36,050,265 | TGGGACGGTTAAGCGTAACACAGTTATGA |
| TUR | 36,050,269 | 36,050,289 | CACAGGGCTGACCTTGATTAC |
| TDF | 36,079,516 | 36,079,538 | TGATGACGAACATGAGTGGATTG |
| TDP | 36,079,540 | 36,079,559 | CAGGACCTGCCGCAAGGGCT |
| TDR | 36,079,565 | 36,079,585 | GGACTTCTGTTTGGGCATTGG |
PCR Primers
| Primer | Sequence |
| SU | CCTGACCAGGTGCCCTCG |
| SD | GTCCCACTTCTCTGAGCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18053A-D8 | VGB6 | C57BL/6NTac | |
| 18053A-A4 | VGB6 | C57BL/6NTac | |
| 18053A-F1 | VGB6 | C57BL/6NTac | |
