| Gene | Nrbp1 |
| Chromosome | 5 |
| Deletion Start | 31,251,183 |
| Deletion End | 31,243,785 |
| Deletion Size | 7,399 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,243,804 | 31,243,824 | CAGTAGTCAGCAGTGGCTCAG |
| TUP | 31,243,834 | 31,243,863 | TAGAATCCTCATCTTTAGCTCCTGGCCTGA |
| TUR | 31,243,876 | 31,243,898 | TGAAGTTGTAGAGGTCACAGGAG |
| TDF | 31,251,079 | 31,251,102 | TTCACCAGGAACAGTACACTCAAC |
| TDP | 31,251,104 | 31,251,126 | CAGCCACTGTCACCGTCTCCTCG |
| TDR | 31,251,159 | 31,251,178 | ATGCCCAGGGACAGCCACAG |
PCR Primers
| Primer | Sequence |
| SU | TTGCCTGTAATGCCTGTACG |
| SD | GCAAGCACAACACAAGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18055A-H11 | VGB6 | C57BL/6NTac | |
| 18055A-C2 | VGB6 | C57BL/6NTac | |
