| Gene | Peg3 |
| Chromosome | 7 |
| Deletion Start | 6,718,438 |
| Deletion End | 6,707,479 |
| Deletion Size | 10,960 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,718,024 | 6,718,047 | GCAGTCTTTAAGTAGCGGGAGTAG |
| TUP | 6,717,995 | 6,718,021 | TGAAGCCACTAAAGAGGGCTGAGATGA |
| TUR | 6,717,940 | 6,717,961 | TCTCACATACAGCCAACCTCTC |
| TDF | 6,707,701 | 6,707,718 | GCCTTTGGCGAGCATCTG |
| TDP | 6,707,670 | 6,707,695 | TCACGCCAGTGTGATCATCTTCGAGC |
| TDR | 6,707,622 | 6,707,641 | TGCTGGCCCGTTCAATGTAG |
PCR Primers
| Primer | Sequence |
| SU | ACAGGCTGCCACAGTCTTTC |
| SD | CCTCTGTGGTCTGGAATTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18056A-H12 | VGB6 | C57BL/6NTac | |
| 18056A-H4 | VGB6 | C57BL/6NTac | |
| 18056A-G8 | VGB6 | C57BL/6NTac | |
| 18056A-E6 | VGB6 | C57BL/6NTac | |
