| Gene | Scaf11 |
| Chromosome | 15 |
| Deletion Start | 96,446,567 |
| Deletion End | 96,414,729 |
| Deletion Size | 31,839 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | CTCTTACTGGCCTGGGAGTTTC |
| TUP | | | TGGCCAGCAAGCTTCAGCAGTTG |
| TUR | | | AGGCTCCGGTGCTGAGAAG |
| TDF | | | GGGCCTGTTGAGTTGGTTCATC |
| TDP | | | AGCACTGACTGCTCTTCCAAAGGTCC |
| TDR | | | GGTCCTGAGCCACCATGTG |
PCR Primers
| Primer | Sequence |
| SU | CGTGCAGCACACCTAACAAC |
| SD | AAATACGCATTTGGGACCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18058A-A8 | VGB6 | C57BL/6NTac | |
| 18058A-C1 | VGB6 | C57BL/6NTac | |
| 18058A-C2 | VGB6 | C57BL/6NTac | |
| 18058A-F9 | VGB6 | C57BL/6NTac | |
| 18058A-D7 | VGB6 | C57BL/6NTac | |
| 18058A-D9 | VGB6 | C57BL/6NTac | |
