| Gene | Nudt21 |
| Chromosome | 8 |
| Deletion Start | 94,036,889 |
| Deletion End | 94,019,593 |
| Deletion Size | 17,297 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,036,173 | 94,036,195 | TTGCAGGCTGTTTTGTTTTCAAC |
| TUP | 94,036,137 | 94,036,166 | TTGTCTTAAGCAGATGATTGCATTTCGGGA |
| TUR | 94,036,049 | 94,036,070 | CAGCTCCACACTAGGGAATGAG |
| TDF | 94,019,902 | 94,019,926 | ATGCCTTCAAGGAACTTTGTGATTC |
| TDP | 94,019,864 | 94,019,886 | TACACTCTTAATGTGTCTCAGGG |
| TDR | 94,019,824 | 94,019,842 | ACCTCACTGGTCCCACTGC |
PCR Primers
| Primer | Sequence |
| SU | AGGCAGTAAAGCACAGGGAG |
| SD | ACACTACTGGAGCAAAGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 103 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18064A-B9 | VGB6 | C57BL/6NTac | |
| 18064B-A11 | VGB6 | C57BL/6NTac | |
| 18064B-C11 | VGB6 | C57BL/6NTac | |
| 18064B-G8 | VGB6 | C57BL/6NTac | |
