| Gene | Hdac3 |
| Chromosome | 18 |
| Deletion Start | 37,954,910 |
| Deletion End | 37,937,594 |
| Deletion Size | 17,317 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,954,744 | 37,954,766 | AGGAGCTGGACACCCAATGAAAC |
| TUP | 37,954,717 | 37,954,741 | CATCGCCTGGCATTGACTCATAGCC |
| TUR | 37,954,670 | 37,954,691 | GCCAACACTCACGATCATCTTC |
| TDF | 37,937,665 | 37,937,685 | AGGCCAGAAGCACCCAATGAG |
| TDP | 37,937,637 | 37,937,663 | TCTATGATGGCGACCATGACAACGACA |
| TDR | 37,937,600 | 37,937,622 | GCATCCATGCTGCTCTAAATCTC |
PCR Primers
| Primer | Sequence |
| SU | CTGCTCTTGCATTCTATGAATG |
| SD | TTGCATGGAAGCAAGAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 18066B-A8 | VGB6 | C57BL/6NTac | |
| 18066B-A11 | VGB6 | C57BL/6NTac | |
| 18066B-D4 | VGB6 | C57BL/6NTac | |
| 18066B-F8 | VGB6 | C57BL/6NTac | |
| 18066B-G5 | VGB6 | C57BL/6NTac | |
| 18066B-G9 | VGB6 | C57BL/6NTac | |
